Simple Summary This study examines the nestedness of anuran species in the Chengdu region, Sichuan, during summers 2019-2020. Using a transect method, the area was surveyed across 23 sites and 8 regions. Habitat data were surveyed with sampling buffers at 1 km, 2 km, and 5 km to assess scale-dependent nestedness. The weighted-nestedness metric (WNODF) revealed strong nested patterns at both site and regional scales. Habitat nestedness was the sole driver, as other factors showed no significant correlation with nestedness. This study recommends habitat diversity protection as a key conservation strategy for anurans in the area.Abstract Nestedness in community ecology predicts that species in a species-poor site should be a subset of species of a species-rich site. A variety of ecological mechanisms have been offered to explain community nestedness; however, few studies have systematically discussed the issue of scale dependence when interpreting community nestedness. This study conducted surveys of anuran species data in the vicinity of Chengdu, Sichuan, in the summers of 2019-2020, using the transect method. The study area was divided into 23 sampling sites and 8 regions to explore the relationship between environmental factors and the nested distribution pattern of anuran communities under different sampling extents (with sampling buffers set at 1 km, 2 km, and 5 km). The WNODF (weighted-nestedness metric based on overlap and decreasing fill) results indicated that anurans exhibited a strong nested pattern at both the sampling sites scale and the regional scale. The habitat matrix test results suggested that a small-scale study area requires a correspondingly small habitat-sampling extent to effectively test for habitat nestedness. As the study area expands, the habitat-sampling range can be appropriately increased. The nested pattern of anurans in the vicinity of Chengdu can only be explained by habitat nestedness, as a Spearman's correlation analysis showed that other environmental factors (area size, connectivity index, concentration index, proximity index, and distance to the city center) were not significantly correlated with the nested sequences of sampling points and regions. Therefore, regarding the conservation strategies for anurans in the vicinity of Chengdu, we recommend prioritizing the protection of areas with higher habitat diversity.