Cyamopsis tetragonoloba (L.), an annual leguminous crop, with wide and important industrial applications. Its seeds contain large amounts of guar gum, main ingredient is galactomannan, have multiple application values. To reveal the genome resource of guar, this study describes the assembly of C. tetragonoloba chromosome-level genome CteV1.0. There were 18.35？Gb high accuracy HiFi data with the more than 40× sequencing depth were generated by the PacBio platform with average length 22.61？kb. Finally, the assembled size of C. tetragonoloba genome was 482.45？Mb with a 62.64？Mb scaffold N50. Then with Hi-C assembly and adjustment, the 99.07% (477.96？Mb/482.45？Mb) of genome data was assigned to 7 pseudochromosomes. A total of 31,251 genes (31251/31867, 98.07%) were successfully annotated, and 309 gene families contracted, 559 gene families expanded in C. tetragonoloba. This genome of guar provides fundamental biological information of the industrial benefits, biophysiological functions and unique evolutionary relationship. The comparison between guar and other related short-day and long-day species characterized the photoperiodic differences in genomic scale. The construction of genome assembly, gene function and photoperiod adaption in C. tetragonoloba may reveal the basic theoretical fundament of the special traits in C. tetragonoloba. This available high-quality genomic resource in this study will facilitate future research in guar field.
This paper was published by JiHan Li, CaiXia Li and XinRong Ma from CIB.