Species are the cornerstone in many domains of biology research, which make accurate species delimitation critically important. In this study, the systematics and biogeography of theHyla chinensisgroup were analyzed based on phylogeny, species delimitation, and ancestral area reconstruction methods. The phylogenetic results showed that six specific clusters existed in theH. chinensisgroup. Bayesian Phylogenetics and Phylogeography (BPP) analysis indicated that six distinct species exist due to the high probability values (>0.95), which were also supported by the Bayes factor (BF) analysis. The divergence time of theH. chinensisgroup was estimated to date back to 18.84 million years ago (Mya) in the early Miocene. Combining the results of ancestral area reconstruction, theH. chinensisgroup might have originated from Guangxi-Hainan, then spread eastwardly and reached Nanling Mountains, Wuyi Mountains, Huangshan Mountain, and Taiwan. In right-about colonization, it was gradually extended to the Yunnan-Guizhou Plateau, Sichuan Basin, Qinling Mountains, and Dabie Mountains. Considering the geological movement from early Miocene to Pliocene, the colonization pattern of theH. chinensisgroup may be closely related to the progressive uplift of the Qinghai-Tibetan Plateau (QTP) and historical climate change. Our study provided evidence for species delimitation and speciation process within theH. chinensisgroup. Our study supported the hypothesis that the evolutionary divergence in this species group was a consequence of the progressive uplift of the QTP and environmental change.