Researchers isolated and characterized ten polymorphic microsatellite loci in the Chong’an moustache toad Leptobrachium liui, nine novel loci designed for this species and one locus from cross-species amplification. Loci were screened in 43 individuals of a single population.

The number of alleles per locus ranged from 3 to 17, on average of 10. The average observed and expected heterozygosity per locus ranged from 0.512 to 0.884 and from 0.580 to 0.898, respectively. No significant departures from Hardy–Weinberg equilibrium were found after sequential Bonferroni corrections. Three loci, CHA8, CHA12, and VIB-D5, were detected to be linked. These polymorphic loci will be useful for conservation genetics and phylogeography studies of this endangered species.

Researchers from Chengdu Institute of Biology conducted this research. And it got supports from National Natural Science Foundation of China Chinese Academy of Sciences.